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Oxford Nanopore native barcoding kit 96 v14
Native Barcoding Kit 96 V14, supplied by Oxford Nanopore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/native+barcoding+kit+96+v14/rapid+barcoding+kit+sqk+rbk004/pm40626692-9-15-8
Average 90 stars, based on 1 article reviews
native barcoding kit 96 v14 - by Bioz Stars, 2026-10
90/100 stars

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Amplification:

Article Title: Impact of the cyanobacterium Synechococcus elongatus PCC 7942 as a dietary supplement on the intestinal microbiota of zebrafish (Danio rerio)
Article Snippet: This study investigated the effects of dietary supplementation with Synechococcus elongatus PCC 7942 on the intestinal microbiota of zebrafish (Danio rerio).. Using metataxonomic analysis based on 16S rRNA gene sequencing, the impact of supplementation on microbial composition and diversity was assessed.. Results revealed that the intestinal microbiota remained generally stable, with no significant changes in alpha (Shannon and Simpson) or beta (Bray–Curtis) diversity indices, suggesting that supplementation did not compromise overall microbial health.

Purification:

Article Title: Influence of Oregano Essential Oil on the Rumen Microbiome of Organically Reared Alpine Goats: Implications for Methanobacteria Abundance.
Article Snippet: .. The obtained amplicons were subsequently purified with AMPure®® XP beads (Beckman Coulter, Brea, CA, USA) and prepared for sequencing through ligation, using the Native Barcoding Kit 96 V14 (SQK-NBD114.96, Oxford Nanopore Technologies plc., Oxford Science Park, Oxford, UK). .. The constructed DNA library containing a pool of 54 barcoded PCR products (27 from each primer pair), was run in a MinION-Mk1B sequencing device with a R.10.4.1 flowcell (Oxford Nanopore Technologies plc., Oxford Science Park, Oxford, UK).

Sequencing:

Article Title: Influence of Oregano Essential Oil on the Rumen Microbiome of Organically Reared Alpine Goats: Implications for Methanobacteria Abundance.
Article Snippet: .. The obtained amplicons were subsequently purified with AMPure®® XP beads (Beckman Coulter, Brea, CA, USA) and prepared for sequencing through ligation, using the Native Barcoding Kit 96 V14 (SQK-NBD114.96, Oxford Nanopore Technologies plc., Oxford Science Park, Oxford, UK). .. The constructed DNA library containing a pool of 54 barcoded PCR products (27 from each primer pair), was run in a MinION-Mk1B sequencing device with a R.10.4.1 flowcell (Oxford Nanopore Technologies plc., Oxford Science Park, Oxford, UK).

Article Title: TITAN-RNA: A hybrid-capture sequencing panel detects known and unknown Flaviviridae for diagnostics and vector surveillance
Article Snippet: Gels were extracted with QIAquick Gel Extraction Kit (Qiagen, catalog# 28704), or RT-PCR products were directly cleaned with QIAquick PCR Purification Kit (Qiagen, catalog# 28104). .. Amplicons were barcoded using the Native Barcoding Kit 96 V14 (Oxford Nanopore; Oxford, UK; cat# SQK-NBD114.96I), prepared libraries were loaded onto a MinION R10.4.1 (FLO-MIN114) flow cells and sequenced with a MinION Mk1b Oxford Nanopore Portable Sequencing device. .. Amplicons were barcoded using the Native Barcoding Kit 96 V14 (Oxford Nanopore; Oxford, UK; cat# SQK-NBD114.96I), prepared libraries were loaded onto a MinION R10.4.1 (FLO-MIN114) flow cells and sequenced with a MinION Mk1b Oxford Nanopore Portable Sequencing device.

Article Title: Sensitivity and Specificity of an All-in-One Cartridge-Based Dengue Real-Time RT-PCR for Point-of-Care Detection and Serotyping of Dengue Virus in Samples From Indonesian Patients.
Article Snippet: RNA was reverse transcribed using Superscript III Reverse Transcriptase (Invitrogen/Thermo Fisher Scientific, Waltham, MA, USA) and amplified using multiplex PCR with Q5 High-Fidelity DNA Polymerase (New England Biolabs, Hitchin, UK). .. The sequencing library was prepared using a Native Barcoding Kit 96 v14 (Oxford Nanopore Technologies) and sequenced using a MinION Mk1B with Flow Cell R v10.4.1 (Oxford Nanopore Technologies). .. The Pod5 data generated using MinKNOW software v23.07.5 (Oxford Nanopore Technologies) were base-called using Guppy v6.5.7 (Oxford Nanopore Technologies) with a super accurate model. Data QC and sequence as- sembly were performed using BBDuk v38.84 and Minimap2 on Geneious Prime v2023.2.1.

Ligation:

Article Title: Influence of Oregano Essential Oil on the Rumen Microbiome of Organically Reared Alpine Goats: Implications for Methanobacteria Abundance.
Article Snippet: .. The obtained amplicons were subsequently purified with AMPure®® XP beads (Beckman Coulter, Brea, CA, USA) and prepared for sequencing through ligation, using the Native Barcoding Kit 96 V14 (SQK-NBD114.96, Oxford Nanopore Technologies plc., Oxford Science Park, Oxford, UK). .. The constructed DNA library containing a pool of 54 barcoded PCR products (27 from each primer pair), was run in a MinION-Mk1B sequencing device with a R.10.4.1 flowcell (Oxford Nanopore Technologies plc., Oxford Science Park, Oxford, UK).

other:

Article Title: A rapid and inexpensive universal PCR protocol for DNA (meta)barcoding using a one-tube, 2-step PCR
Article Snippet: To maximize the cost and effectiveness of barcoding approaches, we developed barcodes based on the Native Barcoding Kit 96 V14 of Oxford Nanopore Technologies (ONT).

Virus:

Article Title: Phylogenetic analysis of H5N1 influenza viruses isolated from dairy cattle in Texas in December 2024.
Article Snippet: .. The virus stocks were then sequenced on the Oxford Nanopore MinION flow cell (FLO-MIN114) using Native Barcoding Kit 96 V14 (SQK-NBD114.96). ..



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